477 posters, 14 topics, 2,065 authors, 1,055 institutions
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The Joint EBJIS-MSIS 2026 Conference
17 - 19 September, 2026 | Porto, Portugal
EP019
Post-operative joint wound fluid is alkaline and suppresses S. aureus metabolism and virulence
Poster Presenter
Authors
Noreen Hickok, Asia Winslow, Emma Dale, Timothy Michaels, Olivia Swiber, Megan Seraydarian, Rachel Butler, Antonia Chen, Christopher Snyder, Thomas Schaer
Affiliations
University of Pennsylvania School of Veterinary Medicine, Kennett Square, United States, UT Southwestern Medical Center, Dallas, United States, Thomas Jefferson University, Thomas Jefferson University, United States
Part of Topic
Basic Science
Introduction:
§ During surgery, the joint space is lavaged, removing the majority of resident synovial fluid.
§ Post-surgery, the joint space is rapidly occupied by a serosanguinous fluid (joint wound fluid) that lacks the proteoglycan-rich (e.g. hyaluronic acid, lubricin), viscous environment of synovial fluid.
v We evaluated the effects of joint wound fluid and its alkaline pH on Staphylococcus aureus metabolism and antibiotic tolerance to better tailor peri-operative antibiotic prophylaxis
Conclusions:
•ovine Wound Joint Fluid decreased S. aureus growth and agr-P3 expression.
•VAN shows time-dependent killing of bacteria in oWJF.
•The aminoglycoside AMK shows rapid bacterial killing in oJWF.
•We suggest that aminoglycosides, which retain efficacy across a broader pH range, warrant greater consideration for local PJI treatment, especially when required for longer than 24 h.