Development of an Aptamer-Based Surface-Enhanced Raman Spectroscopy (SERS) Sensor for the Quantitative Analysis of Fluoride ions

 

Dihan Lakthila, Jayani Jayamanna, Gayathri N. Silva, Neranga Abeyasinghe

Department of Chemistry, University of Colombo, Sri Lanka

Development of a simple and sensitive method for trace-level fluoride detection for healthcare, industrial, and environmental applications.

A DNA fluoride aptamer derived from an RNA fluoride riboswitch was selected as the recognition element to obtain the desired selectivity and a surface-enhanced Raman spectroscopy-based method was chosen to obtain the desired sensitivity for trace-level detection.

As the SERS substrate, Silver nanoparticles were chosen because they possess the highest reported SERS enhancement. 

 

A linear relationship between the fluoride concentration and the Methylene Blue SERS peak around 1600 cm^-1 for fluoride concentrations, within the range of 80 to 120 pM with an R² value of 0.98. 

 

AlphaFold3

GROMACS

GNINA

 

On-chip-biosensor