171 posters, 10 topics, 5 sessions, 690 authors, 211 institutions
ePostersLive by SciGen Technologies S.A. All rights reserved.
American Association of Plastic Surgeons Meeting 2022
April 9 - 12 | San Diego, California
P27
The Ideal Degree Of Dilution For Povidone-iodine As A Breast Implant Irrigant Solution: An In-vitro Model
Part of Topic
Basic Science Research
The Ideal Degree of Dilution for Povidone-Iodine as a Breast Implant Irrigant Solution: An in-Vitro Model
M Ha1; LM Ngaage1,2; RD Smith3; JR Izac3; S Slezak1; RK Ernst3; J Harro3; YM Rasko1
1 Division of Plastic Surgery, Department of Surgery, University of Maryland School of Medicine, Baltimore. 2 Department of Plastic and Reconstructive Surgery, Johns Hopkins University, Baltimore 3 Department of Microbial Pathogenesis, School of Dentistry, University of Maryland Baltimore, Baltimore, Maryland, USA
Background
•In implant-based breast surgery, infections remain a challenging complication, leading to significant clinical and financial consequences.
•Surgeons may choose to prophylactically address this risk by irrigating the implant with povidone-iodine at the time of placement.
•There remains little consensus regarding the ideal dilution of povidione-iodine for optimal efficacy.
Objective
•To assess the effect of dilution of povidone-iodine in prophylactic irrigation of breast implants against Gram-positive and Gram-negative bacteria
Methods
•The authors assessed the efficacy of 10% povidone-iodine at three potential dilutions (100%, 50% or 25% strength) against four groups of bacteria:
•Staphylococcus aureus
•Staphylococcus epidermidis
•Escherichia coli
•Pseudomonas aeruginosa
•Normal saline was included as a negative control. Sterile, smooth implant disks were immersed in each irrigant solution and then incubated in suspensions of each bacterial strain overnight at 37°C.
•Each disk was rinsed and sonicated to displace biofilm forming bacteria.
•The displaced bacteria were quantified, and normalized values were calculated for the bacterial counts.
Results
•Across all strains of both Gram-positive and Gram-negative bacteria, povidone-iodine resulted in lower reduction of bacterial load at greater levels of dilution.
•Povidone-iodine had the greatest reduction in bacterial load in Staphylococcus epidermidis, but the lowest reduction in Pseudomonas aeruginosa.
•A dilution of 25% led to a moderate bacterial load reduction of 103 to 104 in three of our bacterial strains, but no significant reduction in Pseudomonas aeruginosa.
•For 50% and 100% strength, we noted a reduction of 101-105 of all bacterial counts, with no significant difference between the dilutions.
Conclusions
•As we strive to conserve resources in breast surgery, we can recommend that diluting povidone-iodine in equal parts saline does not reduce the efficacy of its anti-microbial properties.