This scientific poster, prepared for the AAIC 26 conference, investigates the acute dose- and time-dependent effects of intranasal insulin on neurodegenerative markers in Wistar rats.

Key Findings

• Rapid, Short-Lived Signaling: A single dose of intranasal insulin triggers a highly transient phosphorylation response in the brain's temporal cortex, peaking at just 3 minutes and fading rapidly.

• No Immediate Effect on Pathology: Acute administration (across multiple doses and time points) failed to alter the levels of core Alzheimer's biomarkers—specifically Amyloid-$\beta$ ($A\beta_{1-42}$) and phosphorylated Tau (p-Tau)—in plasma, CSF, or brain tissue.

• Clinical Takeaway: Because acute, single-dose treatments are too transient to change neurodegenerative pathology, long-term or repeated dosing schedules will be necessary to achieve therapeutic benefits in treating central insulin resistance.