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Osseous Healing of Rat calvarial Defects Augmented with Various Graft Materials and Platelet-rich Fibrin (PRF)
Thursday, March 1 / 6:10 - 6:20 pm / Monitor 10

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Osseous healing of rat calvarial defects augmented with various graft materials and platelet-rich fibrin (PRF)

Nishimura G, Hadaya D, Pi-Anfruns J, Moy PK, Aghaloo TL

Platelet-Rich Fibrin (PRF)
- Second generation platelet concentrate
- Stimulatory bioscaffold
- Contains a reservoir of growth factors beneficial for tissue regeneration
- Has been shown to have a direct effect on angiogenesis
- Often used in combination with bone graft materials to enhance the healing capacity in surgical sites
This study investigates whether PRF has a significant effect on the hard tissue response when added to surgical sites. We further stratified the study by combining PRF with various bone graft materials to evaluate whether PRF had stronger healing capacity with certain materials. MicroCT imaging and analysis, and histological assessment were used to evaluate healing in these sites.

Materials & Methods

Male 3-month old Wistar rats
7mm full-thickness craniotomy defects
Defects implanted with either solitary bone graft material (anorganic bovine bone, allograft cancellous bone, autogenous), PRF alone, or PRF mixed with bone graft material
Animals sacrificed 8 weeks post op for calvarial tissue harvesting
High-resolution microCT machine, Dolphin 3D software, CTAn software, and Image J software used to analyze new bone area, new bone volume, and new bone volume/tissue volume
Calvarial tissue sections were also stained with hematoxylin and eosin (H&E) for histological analysis


-Autogenous bone demonstrated the greatest amount of new bone formation
-Little difference quantitatively between groups that had PRF added to bone graft material
-Little difference quantitatively between groups of solitary bone graft material without PRF
-Largest amount of new bone formation noticed in specimens grafted with autogenous bone
-Histological results show increased vascularity of the bone in calvaria in which PRF membranes were utilized

The addition of PRF to grafted sites does not appear to quantitatively increase new bone formation during early wound healing. However, grafted sites with PRF result in increased vascularity, which is a positive biologic event for bone formation with any grafting procedure.


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