The Effect of Hydrophilic Treatment Surface on Osteoblast Differentiation in Osterix-cherry Mice
Seventy-two, female, 3-month old OSX-mcherry mice were included in the study. Osteoporosis was induced by ovariectomy (OVX) and calcium-deficient diet in 36 mice, test group (TG). The other 36 mice were sham operated (their ovaries were identified and surgically exposed) - control group (CG). Seven weeks following osteoporosis induction, 1 implant (0.9 x 8 mm) was placed in each femur of each animal for both groups (Neodent Implants, Straumann). One implant had a hydrophobic surface (Neoporos) and the other one had a hydrophilic treatment surface (Acqua). New bone formation and bone-implant contact was assessed by histology and Nano CT analysis at 14, 21 and 28 days after implant placement. Calcium content was measured by EDX on the surface of the implant after 7 days. After 3 and 7 days gene expression of several genes were evaluated using real time PCR. The amount of calcium deposited on the surface due to the mineralization process was higher for (Acqua) surface after 7 days. Consistently, in our gene expression studies, hydrophilic treatment surface showed increased levels of gene expression related to bone formation. Analysis in TG showed that genes involved in the bone morphogenetic protein signaling, such as ALP, BSP, SOST and SP7, were significantly activated in the hydrophilic treatment surface.