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Status and highlights of the global DNA barcode reference library for marine peracarids (Crustacea: Peracarida)


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Status and highlights of the global DNA barcode reference library for marine peracarids (Crustacea: Peracarida)

Pedro E Vieira1,2,3*, Henrique Queiroga1 , Filipe O Costa2,3

1Departamento de Biologia and CESAM Centro de Estudos do Ambiente e do Mar, Universidade de Aveiro, 3810 193 Aveiro, Portugal
2CBMA Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, 4710 057 Braga, Portugal
3Institute of Science and Innovation for Bio Sustainability (IB S), University of Minho, Portugal
*Corresponding author: [email protected]

DNA metabarcoding studies rely on reference libraries to accurately assign the sequence data obtained to the correct taxa1


Reference libraries are still incomplete for most marine invertebrates2, namely Peracarida (Fig 1).
Peracarida is a Superorder of the Subphylum Crustacea3
Among the most ecologically important marine benthic invertebrates4


All public peracaridean COI sequences available on BOLDsystems (accessed Dez 2018)

Area: marine and estuarine world waters.
Orders: Amphipoda , Isopoda, Tanaidacea
Quality control step applied:
-minimum length of 500 base pairs
-without stop codons or contamination
-indication of sampling location
-species level taxonomic identification


A total of 6816 DNA barcodes were compiled
The Atlantic Ocean was the one with the highest number of sequences/species and the Indian Ocean with the least (Fig 2)
Amphipoda comprised 80 of the peracaridean morphospecies (Fig 3
571 morphospecies were assigned to 797 BINs (Fig 4)
160 morphospecies split in two or more BINs each total of 386 (Fig 4)
9 morphospecies contributed with 81 BINs (Fig 4)


Only 571 of the more than 10 000 known marine peracarid species 3 are currently represented in BOLD by records
fulfilling our selection criteria

Still a large gap of representative species barcodes from most oceans

High proportion of surplus BINs compared to morphospecies (additional 28 confirms the importance of
library building efforts to unravel suspected hidden diversity and to contribute to tackle the different shortfalls in marine
biodiversity knowledge

The reference library is still very incomplete much more effort needed for its completion and to proceed with the
revision and DNA barcode based probation of the peracaridean fauna


1Leese et al 2016 DNAqua Net Developing new genetic tools for bioassessment and monitoring of aquatic ecosystems in Europe RIO 2 e 11321
2Weigand H et al 2019 DNA barcode reference libraries for the monitoring of aquatic biota in Europe Gap analysis and recommendations for future work Science of The Total Environment 678 499 524
3WoRMS 2019 Peracarida Accessed at http :://marinespecies org/aphia php?p=taxdetails&id= 1090 on 2019-05-02
4Naylor E 1972 British Marine Isopods Keys and Notes for the Identification of the Species Synopses of the British Fauna Academc Press, London New York, 90 pp

Funding sources

This study was supported by the project The NextSea (NORTE 01 0145 FEDER 000032 under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).

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