6th Commonwealth Chemistry Posters

Microfluidic Paper-Based Fluorescent Sensing Device for Onsite Detection of Secnidazole

Shan Arif, Syed Ghulam Musharraf, Dilshad Hussain*, Email: (dilshadhussain@iccs.edu)

HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS) University of Karachi

Background

•Antibiotics play a crucial role in treating bacterial infections and improving global health.

•Secnidazole, a second-generation nitroimidazole, is highly effective in treating bacterial vaginosis, amoebiasis, and giardiasis.

•Traditional analytical methods (HPLC, GC-MS, spectrophotometry) are expensive, time-consuming, and require expertise.

•A rapid, cost-effective, and user-friendly detection platform is needed for secnidazole analysis.

• First-ever development of an N-CDs@N,S-WS₂-based fluorescent sensor for secnidazole detection.

• Microfluidics paper device assisted Smartphone detection for real-time, point-of-care monitoring.

Outcomes

•A microfluidic paper-based device integrated with a smartphone sensing platform was successfully developed.

•Enables real-time, onsite, and instrument-free detection of secnidazole with high sensitivity.

•The N-CDs@N-S-WS₂ composite demonstrated a strong fluorescence quenching effect in the presence of secnidazole.

•Limit of Detection (LOD): 0.117 µM (117 nM) and Limit of Quantification (LOQ): 0.3573 µM (357 nM).

•The sensor effectively detected secnidazole in human serum, demonstrating real-world applicability.

•The detection efficiency of the N-CDs@N,S-WS₂-based fluorescent sensor was validated using UPLC and LCMS.

Microfluidic Paper-Based Fluorescent Sensing Device for Onsite Detection of Secnidazole