253 posters, 14 videos, 27 audios, 14 topics, 392 authors, 132 institutions

4th Commonwealth Chemistry Posters

October 4 - 5, 2023 | Online, United Kingdom

P179

Evaluation of anti-inflammatory activity of ethanol extracts of leaves and bark of Dialium ovoideum Thwaites

View Poster Contact Presenter

Poster Presenter

Upeka Delpachitra

Authors

Upeka Delpachitra, Dinusha Udukala, Medha Gunaratna

Affiliations

Institute of Chemistry Ceylon, College of Chemical Sciences, Rajagiriya, Sri Lanka, Department of Chemistry, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka

Part of Topic

Good health and well-being

Audio

Evaluation of anti-inflammatory activity of ethanol extracts of leaves and bark of Dialium ovoideum Thwaites

Chathurangani D.A.U.¹, Dinusha N. Udukala¹, and Medha J. Gunaratna²

¹Institute of Chemistry Ceylon, College of Chemical Sciences, Rajagiriya, Sri Lanka.

²Department of Chemistry, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka.

upeka@ichemc.edu.lk

Introduction

What is inflammation?

The development of potent anti-inflammatory drugs from natural products with fewer side effects has gained more attention than synthetic drugs.

The medicinal plant used and why?

Dialium ovoideum Thwaites, an endemic plant in Sri Lanka, is used in traditional medicine to treat skin infections, clean wounds, and act as an antidote for snake bites.

Objective

To evaluate the anti-inflammatory effects of ethanol extracts of the leaves and bark of D. ovoideum Thwaites.

Methadology

Preparation of plant extracts

Plant materials were cleaned, washed, shade-dried and ground into fine powder.

Leaves – Cold extraction using sequential extraction method

Bark – Soxhlet extraction method

Human red blood cell (HRBC) membrane stabilization assay

A 1 mL standard/ sample was mixed with 0.1 mL of 10% red blood cell suspension

Incubated at 56ºC for 30 mins

Samples were cooled to RT and centrifuged

The absorbance of the samples was measured at 560 nm using a plate reader

Standard – Aspirin

Control – Red blood cell suspension

Results

% Inhibition =[(Acontrol- Asample)/(A control)]×100%,

Where A = Absorbance

Expressed as IC50 values

Conclusion

The present study demonstrates significant (p<0.05) anti-inflammatory properties of ethanol extracts of the leaves and bark of D. ovoideum Thwaites.

These plant extracts can be used to isolate active compounds that exhibit anti-inflammatory activities and develop value-added products to treat inflammation.

References

Bulugahapitiya, V. P.; Rathnaweera, T. N.; Manawadu, H. C. Phytochemical Composition and Anti-Oxidant Properties of Dialium Ovoideum thwaites (Gal Siyambala) Leaves. Int. J. Minor Fruits, Med. Aromat. Plants. 2020, 6 (1), 13–19.

Bulugahapitiya, V. P.; Rathnaweera, T. N.; Wijayaratne, W. M. D. G. B.; Manawadu, H. C. Cytotoxicity and Antimicrobial Activity of Dialium Ovoideum thwaites , an Endemic Plant in Sri Lanka. Asian J. of Med. Biol. Res. 2020, 6 (2), 316–320.

Uduwela, U. D. H. K.; Deraniyagala, S. A.; Thiripuranathar, G. Antioxidant and Anti-Inflammatory Potential of the Aqueous Extract of the Peel of a Sri Lankan Variety. World J. Pharm. Res. 2019, 8 (2), 154–166.