Seeding Keratinocytes on Papillary and Reticular Dermal Layers Remodels Skin Explants Differently: Towards Improved Skin Grafting
Bage T, Eydmann T, Metcalfe A, Dheansa B, Mbundi L
In full-thickness wounds such a severe burns, complex interactions between our cells and the extracellular matrixbecome disrupted. Untreated, this leads to seriousclinical sequelae.
Previous research in has highlighted differences between the effect of the papillary and reticular dermis in wound healing, suggesting that this could lead to possible optimisation1,2.
However, these studies were conducted in 3D skin models, not skin explants1,2.
Aims of the Study
All images 5x magnification
Firstly, the results show that the two layers of the dermis modulate the repair process of skin in different ways. Since the principal cell type of the dermal compartments is fibroblasts, these findings suggest that the distinction between reticular and papillary fibroblasts is important in wound healing and repair.
In full-thickness wounds where the basement membrane has been compromised, the findings from this study suggest that papillary fibroblasts could be seeded in advance of a cultured epithelial autograft, to promote basement membrane reformation and support keratinocyte differentiation. In contrast, this study suggests that in cases where rapid complete wound coverage is a priority, such as in patients with high total burns surface area, perhaps mixed populations or reticular fibroblasts couldbe employed.
This pilot study is not without its limitations, but it has highlighted exciting areas for further study.