Impact of Antibiotic and Probiotic Intervention on the Gut Microbiome and Resistome of Healthy Adults
A randomized, double-blinded and placebo-controlled clinical trial examined the impact of a two-strain probiotic combination Lacidofil® STRONG (Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0052) on Antibiotic-Associated Diarrhea (AAD) following administration of amoxicillin-clavulanic acid in healthy adults (NCT01941160)1. Metagenomic investigations have reported on the reservoir of antibiotic resistance (ABR) genes present in the gut termed the ‘gut resistome’. These studies also reported on how antibiotic use can cause perturbations in gut resistome and microbiome community diversity and abundance. Various health benefits of probiotics have been reported, however, little is known about the impact on the gut resistome and microbiome after antibiotic administration.
The aim of this study was to evaluate the impact of Lacidofil® STRONG following antibiotic administration on the gut microbiome and resistome of 70 healthy participants (a subgroup of the clinical study cohort) using 16S rRNA targeted metagenomic sequencing, as well shotgun sequencing and a custom-designed ABR microarray.