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P01.13

Mimetic peptide selection from phage-display libraries using patient sera evidence epitope structural features for the apex of V3 loop in gp120.


Mimetic peptide selection from phage-display libraries using patient sera evidence epitope structural features for the apex of V3 loop in gp120
Y. Palacios-Rodríguez1*, T. Gazarian1, Abraham S. Majluf-Cruz2, Leonor Huerta2,  K. Gazarian1
1Department of Molecular Biology and Biotechnology, 2Department of Immunology, Institute of Biomedical Research
Mexican National Autonomous University, Mexico City, Mexico.  *Current address: Autonomous University of Mexico City (UACM);  ypalacios@ yahoo.com
I. INTRODUCTION
 
The crown region of the V3 loop in HIV-1 that contains the conserved amino acid sequence GPGR/G is known as the principal neutralizing determinant due to the extraordinary ability of antibodies to this region to neutralize the virus. This region is highly immunogenic and is accessible on the surface of many primary isolates. The aim of the present study was to use polyclonal antibodies from HIV-1 seropositive patients to select V3 crown mimics from random libraries using GPG triad as epitope marker.